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. 2013 Aug 14;93(5-6):208-13.
doi: 10.1016/j.lfs.2013.06.007. Epub 2013 Jun 16.

Effects of alternate-day fasting on high-fat diet-induced insulin resistance in rat skeletal muscle

Kazuhiko Higashida  1 , Eri Fujimoto, Mitsuru Higuchi, Shin Terada
Affiliations

Effects of alternate-day fasting on high-fat diet-induced insulin resistance in rat skeletal muscle

Kazuhiko Higashida et al. Life Sci. .

Abstract

Aims: The purpose of this study was to investigate the effects of alternate-day fasting (ADF) on insulin-stimulated glucose transport activity in skeletal muscle in rats fed a high-fat diet.

Main methods: Male Wistar rats were placed on a high-fat diet (n=24) or standard chow diet (Chow, n=12) for 10weeks. Rats fed the high-fat diet were separated into two groups after 4weeks. One group was subjected to ADF for the subsequent 6weeks (HF-ADF, n=12), and the other group was maintained on an ad libitum diet (HF-AL, n=12). After the 10-week dietary intervention, measurements of insulin-stimulated glucose uptake and insulin tolerance test (ITT) were performed.

Key findings: Whereas the total intra-abdominal fat mass in the HF-AL group was significantly higher than in the Chow and HF-ADF groups, there was no significant difference between the Chow and HF-ADF groups. However, insulin-stimulated glucose uptake in skeletal muscles was significantly lower in both high-fat fed groups than in the Chow group. Muscle GLUT-4 protein content in HF-AL is significantly lower (~30%) than in Chow, and further reduction (~42%) was observed in the HF-ADF group rats. The HF-ADF and HF-AL group rats had less reduction in glycemia than did the Chow group rats during ITT.

Significance: ADF was unable to eliminate high-fat diet-induced muscle insulin resistance, despite a substantial decrease in total intra-abdominal fat mass. This might have resulted from a reduction in GLUT-4 protein in both HF-AL and HF-ADF rats compared to the Chow group.

Keywords: Alternate-day fasting; GLUT-4; Insulin resistance; Intra-abdominal fat; Rat; Skeletal muscle.

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