Weight loss after gastric bypass surgery in human obesity remodels promoter methylation
- PMID: 23583180
- DOI: 10.1016/j.celrep.2013.03.018
Weight loss after gastric bypass surgery in human obesity remodels promoter methylation
Erratum in
- Cell Rep. 2013 May 30;3(5):1755
Abstract
DNA methylation provides a mechanism by which environmental factors can control insulin sensitivity in obesity. Here, we assessed DNA methylation in skeletal muscle from obese people before and after Roux-en-Y gastric bypass (RYGB). Obesity was associated with altered expression of a subset of genes enriched in metabolic process and mitochondrial function. After weight loss, the expression of the majority of the identified genes was normalized to levels observed in normal-weight, healthy controls. Among the 14 metabolic genes analyzed, promoter methylation of 11 genes was normalized to levels observed in the normal-weight, healthy subjects. Using bisulfite sequencing, we show that promoter methylation of PGC-1α and PDK4 is altered with obesity and restored to nonobese levels after RYGB-induced weight loss. A genome-wide DNA methylation analysis of skeletal muscle revealed that obesity is associated with hypermethylation at CpG shores and exonic regions close to transcription start sites. Our results provide evidence that obesity and RYGB-induced weight loss have a dynamic effect on the epigenome.
Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.
Similar articles
-
Weight loss after Roux-En-Y gastric bypass surgery reveals skeletal muscle DNA methylation changes.Clin Epigenetics. 2021 May 1;13(1):100. doi: 10.1186/s13148-021-01086-6. Clin Epigenetics. 2021. PMID: 33933146 Free PMC article.
-
Mechanisms of improved glycaemic control after Roux-en-Y gastric bypass.Dan Med J. 2015 Apr;62(4):B5057. Dan Med J. 2015. PMID: 25872541 Review.
-
An analysis of DNA methylation in human adipose tissue reveals differential modification of obesity genes before and after gastric bypass and weight loss.Genome Biol. 2015 Jan 22;16(1):8. doi: 10.1186/s13059-014-0569-x. Genome Biol. 2015. PMID: 25651499 Free PMC article.
-
Altered promoter methylation of PDK4, IL1 B, IL6, and TNF after Roux-en Y gastric bypass.Surg Obes Relat Dis. 2014 Jul-Aug;10(4):671-8. doi: 10.1016/j.soard.2013.12.019. Epub 2014 Jan 28. Surg Obes Relat Dis. 2014. PMID: 24837562
-
PGC-1α and exercise in the control of body weight.Int J Obes (Lond). 2012 Nov;36(11):1428-35. doi: 10.1038/ijo.2012.12. Epub 2012 Jan 31. Int J Obes (Lond). 2012. PMID: 22290535 Review.
Cited by
-
Transcriptional and epigenetic changes after dietary and surgical weight loss interventions in an animal model of obesity.Int J Obes (Lond). 2024 Jan;48(1):103-110. doi: 10.1038/s41366-023-01395-1. Epub 2023 Oct 13. Int J Obes (Lond). 2024. PMID: 37833561
-
Effects of obesity, and of weight loss following bariatric surgery, on methylation of DNA from the rectal mucosa and in cell-free DNA from blood.Int J Obes (Lond). 2023 Dec;47(12):1278-1285. doi: 10.1038/s41366-023-01384-4. Epub 2023 Sep 15. Int J Obes (Lond). 2023. PMID: 37714902
-
Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry.Medicina (Kaunas). 2023 Jul 15;59(7):1313. doi: 10.3390/medicina59071313. Medicina (Kaunas). 2023. PMID: 37512124 Free PMC article.
-
New Insights and Potential Therapeutic Interventions in Metabolic Diseases.Int J Mol Sci. 2023 Jun 26;24(13):10672. doi: 10.3390/ijms241310672. Int J Mol Sci. 2023. PMID: 37445852 Free PMC article. Review.
-
DNA methylation is differentially associated with glycemic outcomes by different types of weight-loss interventions: an epigenome-wide association study.Clin Epigenetics. 2023 Jul 1;15(1):108. doi: 10.1186/s13148-023-01522-9. Clin Epigenetics. 2023. PMID: 37393279 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials