Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Dec;43(6):2561-7.
doi: 10.1007/s00726-012-1312-0. Epub 2012 May 8.

Glutamine stimulates mTORC1 independent of the cell content of essential amino acids

Martina Chiu  1 , Saverio Tardito, Amelia Barilli, Massimiliano G Bianchi, Valeria Dall'Asta, Ovidio Bussolati
Affiliations

Glutamine stimulates mTORC1 independent of the cell content of essential amino acids

Martina Chiu et al. Amino Acids. 2012 Dec.

Abstract

Glutamine and leucine are important mTORC1 modulators, although their roles are not precisely defined. In HepG2 and HeLa cells glutamine-free incubation lowers mTORC1 activity, although cell leucine is not decreased. mTORC1 activity, suppressed by amino acid-free incubation, is completely rescued only if essential amino acids (EAA) and glutamine are simultaneously restored, although cell leucine is higher in the absence than in the presence of glutamine. Thus, glutamine stimulates mTORC1 independent of cell leucine, suggesting the existence of two distinct stimulatory signals from either glutamine or EAA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • Investigations of potential non-amino acid SNAT2 inhibitors.
    Jakobsen S, Petersen EF, Nielsen CU. Jakobsen S, et al. Front Pharmacol. 2024 Jan 4;14:1302445. doi: 10.3389/fphar.2023.1302445. eCollection 2023. Front Pharmacol. 2024. PMID: 38239202 Free PMC article.
  • Pigeon during the Breeding Cycle: Behaviors, Composition and Formation of Crop Milk, and Physiological Adaptation.
    Wang L, Zhu J, Xie P, Gong D. Wang L, et al. Life (Basel). 2023 Sep 4;13(9):1866. doi: 10.3390/life13091866. Life (Basel). 2023. PMID: 37763270 Free PMC article. Review.
  • Enhanced Antitumor Effect in Liver Cancer by Amino Acid Depletion-Induced Oxidative Stress.
    Okuda K, Umemura A, Kataoka S, Yano K, Takahashi A, Okishio S, Taketani H, Seko Y, Nishikawa T, Yamaguchi K, Moriguchi M, Nakagawa H, Liu Y, Mitsumoto Y, Kanbara Y, Shima T, Okanoue T, Itoh Y. Okuda K, et al. Front Oncol. 2021 Nov 2;11:758549. doi: 10.3389/fonc.2021.758549. eCollection 2021. Front Oncol. 2021. PMID: 34796113 Free PMC article.
  • A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling.
    Fairweather SJ, Okada S, Gauthier-Coles G, Javed K, Bröer A, Bröer S. Fairweather SJ, et al. Front Mol Biosci. 2021 Apr 13;8:646574. doi: 10.3389/fmolb.2021.646574. eCollection 2021. Front Mol Biosci. 2021. PMID: 33928121 Free PMC article.
  • The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
    Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ. Bouhaddou M, et al. Cell. 2020 Aug 6;182(3):685-712.e19. doi: 10.1016/j.cell.2020.06.034. Epub 2020 Jun 28. Cell. 2020. PMID: 32645325 Free PMC article.
See all "Cited by" articles

Publication types

MeSH terms

Substances

LinkOut - more resources