Obesity related methylation changes in DNA of peripheral blood leukocytes
- PMID: 21176133
- PMCID: PMC3016263
- DOI: 10.1186/1741-7015-8-87
Obesity related methylation changes in DNA of peripheral blood leukocytes
Abstract
Background: Despite evidence linking obesity to impaired immune function, little is known about the specific mechanisms. Because of emerging evidence that immune responses are epigenetically regulated, we hypothesized that DNA methylation changes are involved in obesity induced immune dysfunction and aimed to identify these changes.
Method: We conducted a genome wide methylation analysis on seven obese cases and seven lean controls aged 14 to 18 years from extreme ends of the obesity distribution and performed further validation of six CpG sites from six genes in 46 obese cases and 46 lean controls aged 14 to 30 years.
Results: In comparison with the lean controls, we observed one CpG site in the UBASH3A gene showing higher methylation levels and one CpG site in the TRIM3 gene showing lower methylation levels in the obese cases in both the genome wide step (P = 5 × 10(-6) and P = 2 × 10(-5) for the UBASH3A and the TRIM3 gene respectively) and the validation step (P = 0.008 and P = 0.001 for the UBASH3A and the TRIM3 gene respectively).
Conclusions: Our results provide evidence that obesity is associated with methylation changes in blood leukocyte DNA. Further studies are warranted to determine the causal direction of this relationship as well as whether such methylation changes can lead to immune dysfunction.
Figures
Comment in
-
Epigenetics and obesity: the devil is in the details.BMC Med. 2010 Dec 21;8:88. doi: 10.1186/1741-7015-8-88. BMC Med. 2010. PMID: 21176136 Free PMC article.
-
Nutritional status affects the epigenomic profile of peripheral blood cells.Epigenomics. 2011 Jun;3(3):259-60. doi: 10.2217/epi.11.24. Epigenomics. 2011. PMID: 22122335 No abstract available.
Similar articles
-
An epigenome-wide study of obesity in African American youth and young adults: novel findings, replication in neutrophils, and relationship with gene expression.Clin Epigenetics. 2018 Jan 5;10:3. doi: 10.1186/s13148-017-0435-2. eCollection 2018. Clin Epigenetics. 2018. PMID: 29312471 Free PMC article.
-
DNA methylation map in circulating leukocytes mirrors subcutaneous adipose tissue methylation pattern: a genome-wide analysis from non-obese and obese patients.Sci Rep. 2017 Feb 17;7:41903. doi: 10.1038/srep41903. Sci Rep. 2017. PMID: 28211912 Free PMC article.
-
DNA methylation of the LY86 gene is associated with obesity, insulin resistance, and inflammation.Twin Res Hum Genet. 2014 Jun;17(3):183-91. doi: 10.1017/thg.2014.22. Epub 2014 Apr 15. Twin Res Hum Genet. 2014. PMID: 24735745 Free PMC article.
-
A genome-wide methylation study on obesity: differential variability and differential methylation.Epigenetics. 2013 May;8(5):522-33. doi: 10.4161/epi.24506. Epub 2013 Apr 17. Epigenetics. 2013. PMID: 23644594 Free PMC article.
-
Epigenetics and obesity: the devil is in the details.BMC Med. 2010 Dec 21;8:88. doi: 10.1186/1741-7015-8-88. BMC Med. 2010. PMID: 21176136 Free PMC article.
Cited by
-
DNA Methylation Signatures in Paired Placenta and Umbilical Cord Samples: Relationship with Maternal Pregestational Body Mass Index and Offspring Metabolic Outcomes.Biomedicines. 2024 Jan 27;12(2):301. doi: 10.3390/biomedicines12020301. Biomedicines. 2024. PMID: 38397903 Free PMC article.
-
Insights into the role of nucleotide methylation in metabolic-associated fatty liver disease.Front Immunol. 2023 Mar 20;14:1148722. doi: 10.3389/fimmu.2023.1148722. eCollection 2023. Front Immunol. 2023. PMID: 37020540 Free PMC article. Review.
-
Improper preanalytical processes on peripheral blood compromise RNA quality and skew the transcriptional readouts of mRNA and LncRNA.Front Genet. 2023 Jan 4;13:1091685. doi: 10.3389/fgene.2022.1091685. eCollection 2022. Front Genet. 2023. PMID: 36685907 Free PMC article.
-
Drastic transformation of visceral adipose tissue and peripheral CD4 T cells in obesity.Front Immunol. 2023 Jan 4;13:1044737. doi: 10.3389/fimmu.2022.1044737. eCollection 2022. Front Immunol. 2023. PMID: 36685567 Free PMC article. Review.
-
Immune response to arbovirus infection in obesity.Front Immunol. 2022 Nov 18;13:968582. doi: 10.3389/fimmu.2022.968582. eCollection 2022. Front Immunol. 2022. PMID: 36466818 Free PMC article. Review.
References
-
- Poirier P, Giles TD, Bray GA, Hong Y, Stern JS, Pi-Sunyer FX, Eckel RH. Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss: an update of the 1997 American Heart Association Scientific Statement on Obesity and Heart Disease from the Obesity Committee of the Council on Nutrition, Physical Activity, and Metabolism. Circulation. 2006;113:898–918. doi: 10.1161/CIRCULATIONAHA.106.171016. - DOI - PubMed
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical