Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
- PMID: 20566857
- PMCID: PMC2900693
- DOI: 10.1073/pnas.1002601107
Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
Abstract
Upon delivery, the neonate is exposed for the first time to a wide array of microbes from a variety of sources, including maternal bacteria. Although prior studies have suggested that delivery mode shapes the microbiota's establishment and, subsequently, its role in child health, most researchers have focused on specific bacterial taxa or on a single body habitat, the gut. Thus, the initiation stage of human microbiome development remains obscure. The goal of the present study was to obtain a community-wide perspective on the influence of delivery mode and body habitat on the neonate's first microbiota. We used multiplexed 16S rRNA gene pyrosequencing to characterize bacterial communities from mothers and their newborn babies, four born vaginally and six born via Cesarean section. Mothers' skin, oral mucosa, and vagina were sampled 1 h before delivery, and neonates' skin, oral mucosa, and nasopharyngeal aspirate were sampled <5 min, and meconium <24 h, after delivery. We found that in direct contrast to the highly differentiated communities of their mothers, neonates harbored bacterial communities that were undifferentiated across multiple body habitats, regardless of delivery mode. Our results also show that vaginally delivered infants acquired bacterial communities resembling their own mother's vaginal microbiota, dominated by Lactobacillus, Prevotella, or Sneathia spp., and C-section infants harbored bacterial communities similar to those found on the skin surface, dominated by Staphylococcus, Corynebacterium, and Propionibacterium spp. These findings establish an important baseline for studies tracking the human microbiome's successional development in different body habitats following different delivery modes, and their associated effects on infant health.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Comment in
-
Additional maternal and nonmaternal factors contribute to microbiota shaping in newborns.Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):E159; author reply E160. doi: 10.1073/pnas.1010526107. Epub 2010 Sep 27. Proc Natl Acad Sci U S A. 2010. PMID: 20876088 Free PMC article. No abstract available.
Similar articles
-
Microbiome analysis of maternal and neonatal microbial communities associated with the different delivery modes based on 16S rRNA gene amplicon sequencing.Taiwan J Obstet Gynecol. 2023 Sep;62(5):687-696. doi: 10.1016/j.tjog.2023.07.033. Taiwan J Obstet Gynecol. 2023. PMID: 37678996
-
Gut Microbiota Composition in Healthy Japanese Infants and Young Adults Born by C-Section.Ann Nutr Metab. 2018;73 Suppl 3:4-11. doi: 10.1159/000490841. Epub 2018 Jul 24. Ann Nutr Metab. 2018. PMID: 30041174 Review.
-
Maturation of the infant microbiome community structure and function across multiple body sites and in relation to mode of delivery.Nat Med. 2017 Mar;23(3):314-326. doi: 10.1038/nm.4272. Epub 2017 Jan 23. Nat Med. 2017. PMID: 28112736 Free PMC article.
-
The mode of delivery affects the diversity and colonization pattern of the gut microbiota during the first year of infants' life: a systematic review.BMC Gastroenterol. 2016 Jul 30;16(1):86. doi: 10.1186/s12876-016-0498-0. BMC Gastroenterol. 2016. PMID: 27475754 Free PMC article. Review.
-
Partial restoration of the microbiota of cesarean-born infants via vaginal microbial transfer.Nat Med. 2016 Mar;22(3):250-3. doi: 10.1038/nm.4039. Epub 2016 Feb 1. Nat Med. 2016. PMID: 26828196 Free PMC article.
Cited by
-
The association between caesarean section delivery and obesity at age 17 years. Evidence from a longitudinal cohort study in the United Kingdom.PLoS One. 2024 May 31;19(5):e0301684. doi: 10.1371/journal.pone.0301684. eCollection 2024. PLoS One. 2024. PMID: 38820521 Free PMC article.
-
Unveiling the gut-eye axis: how microbial metabolites influence ocular health and disease.Front Med (Lausanne). 2024 May 10;11:1377186. doi: 10.3389/fmed.2024.1377186. eCollection 2024. Front Med (Lausanne). 2024. PMID: 38799150 Free PMC article. Review.
-
Human Milk Microbiome-A Review of Scientific Reports.Nutrients. 2024 May 8;16(10):1420. doi: 10.3390/nu16101420. Nutrients. 2024. PMID: 38794658 Free PMC article. Review.
-
Transmission and Persistence of Infant Gut-Associated Bifidobacteria.Microorganisms. 2024 Apr 27;12(5):879. doi: 10.3390/microorganisms12050879. Microorganisms. 2024. PMID: 38792709 Free PMC article. Review.
-
Current Knowledge on the Preparation and Benefits of Cruciferous Vegetables as Relates to In Vitro, In Vivo, and Clinical Models of Inflammatory Bowel Disease.Curr Dev Nutr. 2024 Apr 17;8(5):102160. doi: 10.1016/j.cdnut.2024.102160. eCollection 2024 May. Curr Dev Nutr. 2024. PMID: 38779039 Free PMC article. Review.
References
-
- Zhou X, et al. Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J. 2007;1:121–133. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous