Estradiol regulation of proopiomelanocortin gene expression and peptide content in the hypothalamus
- PMID: 1620284
- DOI: 10.1159/000126111
Estradiol regulation of proopiomelanocortin gene expression and peptide content in the hypothalamus
Abstract
Previous studies have shown that the hypothalamic concentrations of beta-endorphin (beta-EP) and other proopiomelanocortin (POMC)-derived peptides change in the female rat following castration and gonadal steroid replacement. In this study we have measured POMC mRNA by solution hybridization assay in the medial basal hypothalamus (MBH) of ovariectomized rats treated with a regimen of estradiol (E2) that we have previously shown alters brain beta-EP peptide content. In addition the effect of progesterone (P) was also studied. In the first experiment the concentration of beta-EP and alpha-melanocyte-stimulating hormone (alpha-MSH) in the MBH of castrated rats decreased significantly after 3 weeks of E2 treatment compared to castrated unreplaced rats: beta-EP decreased from 6.00 +/- 0.46 to 4.32 +/- 0.38 ng/mg protein and alpha-MSH decreased from 3.00 +/- 0.23 to 2.35 +/- 0.15 ng/mg protein (p less than 0.05). A similar decrease in peptide content was noted in the anterior hypothalamus/preoptic area. A parallel reduction in the concentration of POMC mRNA was measured in the MBH of the E2-replaced animals: 1.17 +/- 0.14 vs. 0.72 +/- 0.08 pg/microgram RNA (p less than 0.02). In a second study castrated rats were studied after 2 weeks of E2 or E2 plus P treatment. After 2 weeks, POMC peptide levels did not change significantly in the MBH of either the E2- or E2 plus P-treated rats. POMC mRNA, however, was significantly reduced from 1.10 +/- 0.10 pg/micrograms RNA in the unreplaced rats to 0.58 +/- 0.05 and 0.61 +/- 0.06 pg/microgram RNA after E2 or E2 plus P, respectively (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Obesity and the hypothalamus: novel peptides for new pathways.Cell. 1998 Feb 20;92(4):437-40. doi: 10.1016/s0092-8674(00)80937-x. Cell. 1998. PMID: 9491885 Review. No abstract available.
-
Effect of morphine on proopiomelanocortin gene expression and peptide levels in the hypothalamus.Brain Res Mol Brain Res. 1996 Sep 5;41(1-2):140-7. doi: 10.1016/0169-328x(96)00084-8. Brain Res Mol Brain Res. 1996. PMID: 8883945
-
Dopamine and sex steroid regulation of POMC gene expression in the hypothalamus.Neuroendocrinology. 1993 Nov;58(5):493-500. doi: 10.1159/000126582. Neuroendocrinology. 1993. PMID: 8115018
-
Analysis of steroid action on gene expression in the brain.Ann N Y Acad Sci. 1986;474:453-60. doi: 10.1111/j.1749-6632.1986.tb28035.x. Ann N Y Acad Sci. 1986. PMID: 3555245 Review. No abstract available.
-
Androgen regulation of proopiomelanocortin gene expression and peptide content in the basal hypothalamus.Endocrinology. 1989 May;124(5):2283-8. doi: 10.1210/endo-124-5-2283. Endocrinology. 1989. PMID: 2523303
Cited by
-
Estradiol and Mu opioid-mediated reward: The role of estrogen receptors in opioid use.Addict Neurosci. 2023 Dec 15;9:100139. doi: 10.1016/j.addicn.2023.100139. Epub 2023 Nov 25. Addict Neurosci. 2023. PMID: 38155959 Free PMC article.
-
Ovariectomy, but not orchiectomy, exacerbates metabolic syndrome after maternal high-fructose intake in adult offspring.Korean J Physiol Pharmacol. 2021 Jan 1;25(1):39-49. doi: 10.4196/kjpp.2021.25.1.39. Korean J Physiol Pharmacol. 2021. PMID: 33361536 Free PMC article.
-
Estrogen administration improves the trajectory of eating disorder pathology in oligo-amenorrheic athletes: A randomized controlled trial.Psychoneuroendocrinology. 2019 Apr;102:273-280. doi: 10.1016/j.psyneuen.2018.11.013. Epub 2018 Nov 16. Psychoneuroendocrinology. 2019. PMID: 30639922 Free PMC article. Clinical Trial.
-
The Arcuate Estrogen-Regulated Transcriptome: Estrogen Response Element-Dependent and -Independent Signaling of ERα in Female Mice.Endocrinology. 2017 Mar 1;158(3):612-626. doi: 10.1210/en.2016-1663. Endocrinology. 2017. PMID: 28359086 Free PMC article.
-
Gene Expression and the Control of Food Intake by Hypothalamic POMC/CART Neurons.Open Neuroendocrinol J. 2010;3:21-27. Epub 2010 Feb 22. Open Neuroendocrinol J. 2010. PMID: 28042349 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous