Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54
- PMID: 15375028
- DOI: 10.1210/en.2004-0836
Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54
Abstract
Loss-of-function mutations of the gene encoding GPR54, the putative receptor for the KiSS-1-derived peptide metastin, have been recently associated with hypogonadotropic hypogonadism, in both rodents and humans. Yet the actual role of the KiSS-1/GPR54 system in the neuroendocrine control of gonadotropin secretion remains largely unexplored. To initiate such analysis, the effects of KiSS-1 peptide on LH secretion were monitored using in vivo and in vitro settings under different experimental conditions. Central intracerebroventricular administration of KiSS-1 peptide potently elicited LH secretion in vivo over a range of doses from 10 pmol to 1 nmol. The effect of centrally injected KiSS-1 appeared to be mediated via the hypothalamic LHRH. However, no effect of central administration of KiSS-1 was detected on relative LHRH mRNA levels. Likewise, systemic (i.p. and i.v.) injection of KiSS-1 markedly stimulated LH secretion. This effect was similar in terms of maximum response to that of central administration of KiSS-1 and might be partially attributed to its ability to stimulate LH secretion directly at the pituitary. Finally, the LH-releasing activity of KiSS-1 was persistently observed after blockade of endogenous excitatory amino acid and nitric oxide pathways, i.e. relevant neurotransmitters in the neuroendocrine control of LH secretion. In summary, our results provide solid evidence for a potent stimulatory effect of KiSS-1 on LH release, acting at central levels (likely the hypothalamus) and eventually at the pituitary, and further document a novel role of the KiSS-1/GPR54 system as a relevant downstream element in the neuroendocrine network governing LH secretion.
Similar articles
-
Regulation of the neuroendocrine reproductive axis by kisspeptin-GPR54 signaling.Reproduction. 2006 Apr;131(4):623-30. doi: 10.1530/rep.1.00368. Reproduction. 2006. PMID: 16595713 Review.
-
KiSS-1 and GPR54 as new players in gonadotropin regulation and puberty.Endocrine. 2005 Apr;26(3):277-84. doi: 10.1385/ENDO:26:3:277. Endocrine. 2005. PMID: 16034182 Review.
-
Effects of KiSS-1 peptide, the natural ligand of GPR54, on follicle-stimulating hormone secretion in the rat.Endocrinology. 2005 Apr;146(4):1689-97. doi: 10.1210/en.2004-1353. Epub 2005 Jan 6. Endocrinology. 2005. PMID: 15637288
-
Advanced vaginal opening and precocious activation of the reproductive axis by KiSS-1 peptide, the endogenous ligand of GPR54.J Physiol. 2004 Dec 1;561(Pt 2):379-86. doi: 10.1113/jphysiol.2004.072298. Epub 2004 Oct 14. J Physiol. 2004. PMID: 15486019 Free PMC article.
-
Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormone-releasing activity of KiSS-1 peptide.Endocrinology. 2004 Oct;145(10):4565-74. doi: 10.1210/en.2004-0413. Epub 2004 Jul 8. Endocrinology. 2004. PMID: 15242985
Cited by
-
Human-specific epigenomic states in spermatogenesis.Comput Struct Biotechnol J. 2023 Dec 27;23:577-588. doi: 10.1016/j.csbj.2023.12.037. eCollection 2024 Dec. Comput Struct Biotechnol J. 2023. PMID: 38274996 Free PMC article.
-
The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo.Front Endocrinol (Lausanne). 2023 Oct 12;14:1269334. doi: 10.3389/fendo.2023.1269334. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 37900144 Free PMC article.
-
Is There an Association Between Circulating Kisspeptin Levels and Ovarian Reserve in Women of Reproductive Age?In Vivo. 2023 Sep-Oct;37(5):2219-2223. doi: 10.21873/invivo.13322. In Vivo. 2023. PMID: 37652519 Free PMC article.
-
The kisspeptin system in and beyond reproduction: exploring intricate pathways and potential links between endometriosis and polycystic ovary syndrome.Rev Endocr Metab Disord. 2024 Apr;25(2):239-257. doi: 10.1007/s11154-023-09826-0. Epub 2023 Jul 28. Rev Endocr Metab Disord. 2024. PMID: 37505370 Review.
-
Precocious puberty under stressful conditions: new understanding and insights from the lessons learnt from international adoptions and the COVID-19 pandemic.Front Endocrinol (Lausanne). 2023 May 2;14:1149417. doi: 10.3389/fendo.2023.1149417. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 37201098 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases