Cryo-EM structure of the yeast 80S ribosome with tigecycline, eEF2, Stm1 and eIF5A
Li X, Wang M, Denk T, Buschauer R, Li Y, Beckmann R, Cheng J
Nat Commun (2024) 15 pp. 5481-5481 [ DOI: doi:10.1038/s41467-024-49797-7 Pubmed: 38942792 ]
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us14a (6 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es19a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae)
- Elongation factor 2 (93 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el18a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul29a (13 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein el38 (8 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el41a (3 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us13a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el36a (11 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul4a (39 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el42a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us19 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el19a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul10 (33 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul23 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es17a (15 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor protein stm1 (30 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul2a (27 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es10a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome (Complex from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul14a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el31a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el37a (9 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es21a (9 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es26b (13 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein es31 fusion protein (17 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul5a (19 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es28a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el24a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el29 (6 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein rack1 (34 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 5a-1 (17 kDa, Protein from Saccharomyces cerevisiae)
- Tigecycline (587 Da, Ligand)
- Large ribosomal subunit protein el34a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el32 (14 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein ul24a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el43a (10 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el14a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul22a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el21a (18 kDa, Protein from Saccharomyces cerevisiae)
- 23s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el6a (20 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us2a (28 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul18 (33 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul16 (25 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el22a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul30a (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el33a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es25a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us17a (17 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us9a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul11a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el27a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein el40a fusion protein (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast 80S ribosome with tigecycline, Not5 and P-site tRNA
Li X, Wang M, Denk T, Buschauer R, Li Y, Beckmann R, Cheng J
Nat Commun (2024) 15 pp. 5481-5481 [ DOI: doi:10.1038/s41467-024-49797-7 Pubmed: 38942792 ]
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us14a (6 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es19a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el18a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul29a (13 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein el38 (8 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el41a (3 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us13a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el36a (11 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul4a (39 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el42a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us19 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el19a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul10 (33 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul23 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es17a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul2a (27 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es10a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome (Complex from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul14a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el31a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el37a (9 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es21a (9 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es26b (13 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein es31 fusion protein (17 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul5a (19 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es28a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el24a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el29 (6 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein rack1 (34 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- Tigecycline (587 Da, Ligand)
- Large ribosomal subunit protein el34a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el32 (14 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein ul24a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el43a (10 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el14a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul22a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el21a (18 kDa, Protein from Saccharomyces cerevisiae)
- 23s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el6a (20 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us2a (28 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul18 (33 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul16 (25 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el22a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul30a (27 kDa, Protein from Saccharomyces cerevisiae)
- General negative regulator of transcription subunit 5 (65 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el33a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es25a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us17a (17 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us9a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul11a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el27a (15 kDa, Protein from Saccharomyces cerevisiae)
- Spermidine (145 Da, Ligand)
- Trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein el40a fusion protein (14 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (935 Da, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae)
Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K
Mol Cell (2024) [ DOI: doi:10.1016/j.molcel.2024.05.031 Pubmed: 38925115 ]
Hir3 Arm/Tail, Hir2 WD40, Hpc2 C-term
Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K
Mol Cell (2024) [ DOI: doi:10.1016/j.molcel.2024.05.031 Pubmed: 38925115 ]
Hir1 WD40 domains and Asf1/H3/H4
Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K
Mol Cell (2024) [ DOI: doi:10.1016/j.molcel.2024.05.031 Pubmed: 38925115 ]
Composite map of the Hir complex with Asf1/H3/H4
Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K
Mol Cell (2024) [ DOI: doi:10.1016/j.molcel.2024.05.031 Pubmed: 38925115 ]
Rea1 delta AAA2H2alpha ATPgS structure
Busselez J, Koenig G, Dominique C, Klos T, Velayudhan D, Sosnowski P, Marechal N, Crucifix C, Gizardin-Fredon H, Cianferani S, Benjamin A, Henry Y, Henras A, Schmidt H
To Be Published
- Rea1 monomer (500 kDa, Cellular component from Saccharomyces cerevisiae)
Rea1 D2915A-R2976A-D3042A mutant ATP conformation I
Busselez J, Koenig G, Dominique C, Klos T, Velayudhan D, Sosnowski P, Marechal N, Crucifix C, Gizardin-Fredon H, Cianferani S, Benjamin A, Henry Y, Henras A, Schmidt H
To Be Published
- Rea1 monomer (500 kDa, Cellular component from Saccharomyces cerevisiae)
Rea1 D2915A-R2976A-D3042A ATP conformation II
Busselez J, Koenig G, Dominique C, Klos T, Velayudhan D, Sosnowski P, Marechal N, Crucifix C, Gizardin-Fredon H, Cianferani S, Benjamin A, Henry Y, Henras A, Schmidt H
To Be Published
- Rea1 monomer (500 kDa, Cellular component from Saccharomyces cerevisiae)
Rea1 D2915A-R2976A-D3042A mutant ATP conformation III
Busselez J, Koenig G, Dominique C, Klos T, Velayudhan D, Sosnowski P, Marechal N, Crucifix C, Gizardin-Fredon H, Cianferani S, Benjamin A, Henry Y, Henras A, Schmidt H
To Be Published
- Rea1 monomer (500 kDa, Cellular component from Saccharomyces cerevisiae)