In this work, the ability of S-layer proteins from kefir-isolated Lactobacillus kefir strains to
antagonize the cytophatic effects of toxins from Clostridium difficile (TcdA and TcdB) on
eukaryotic cells in vitro was tested by cell detachment assay. S-layer proteins from eight
different L. kefir strains were able to inhibit the damage induced by C. difficile spent culture
supernatant to Vero cells. Besides, same protective effect was observed by F-actin network
staining. S-layer proteins from aggregating L. kefir strains (CIDCA 83115, 8321, 8345 and�…