Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human�…

GT Macfarlane, S Macfarlane, GR Gibson�- Microbial ecology, 1998 - Springer
… a three-stage compound continuous culture system that enables the activities of intestinal
bacteria … -excess conditions that characterize the proximal colon, as well as the carbohydrate-…

Polysaccharide degradation by human intestinal bacteria during growth under multi-substrate limiting conditions in a three-stage continuous culture system

S Macfarlane, ME Quigley, MJ Hopkins…�- …�Ecology, 1998 - academic.oup.com
… of intestinal bacteria impact on many facets of host metabolism [7,8]. It is … microorganisms
[11,12], and has been validated in … of system retention time on polysaccharide breakdown in the …

Studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using FISH techniques

…, S Macfarlane, GT Macfarlane�- …�ecology, 2006 - academic.oup.com
… on bacterial populations and metabolic events in the proximal … (CCS) used in this study has
been validated previously and … used in this investigation detect a range of bacteria, with the …

Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system

S Macfarlane, EJ Woodmansey…�- Applied and�…, 2005 - Am Soc Microbiol
… were investigated in this study to determine their metabolic capabilities. … 1998. Polysaccharide
degradation by … conditions in a three-stage continuous culture system. FEMS Microbiol. …

Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

D Feria-Gervasio, W Tottey, N Gaci, M Alric…�- Journal of�…, 2014 - Elsevier
… The technical and ethical difficulties in studying the gut … indicated in Table 1: Retention Time
(RT) was calculated as the … course of the continuous culture, the anoxic environment being …

Models for intestinal fermentation: association between food components, delivery systems, bioavailability and functional interactions in the gut

GT Macfarlane, S Macfarlane�- Current opinion in biotechnology, 2007 - Elsevier
… and validated a new three-stage continuous model with … use of a three-stage continuous
culture system to investigate … were greatly lowered when the system retention time was changed …

New three-stage in vitro model for infant colonic fermentation with immobilized fecal microbiota

C Cinquin, G Le Blay, I Fliss…�- …�microbiology ecology, 2006 - academic.oup.com
… and validation of a new three-stage culture system with … that this new three-stage
continuous culture with immobilized … The total mean retention time in the system with a total …

In vitro maintenance of a human proximal colon microbiota using the continuous fermentation system P-ECSIM

D Feria-Gervasio, S Denis, M Alric…�- Applied microbiology and�…, 2011 - Springer
… developed into a three-stage continuous system to simulate the … Here, we propose an
alternative continuous culture system … to validate a three-part continuous fermentation system. This …

Adherence and cytokine induction in Caco-2 cells by bacterial populations from a three-stage continuous-culture model of the large intestine

…, MW Child, S Macfarlane, GT Macfarlane�- Applied and�…, 2011 - Am Soc Microbiol
metabolic activities in different parts of the large bowel, and most … was operated at a total
system retention time of either 20 or 60 h, … Falk PG, Hooper LV, Midtvedt T., and Gordon JI 1998. …

Rifaximin modulates the colonic microbiota of patients with Crohn's disease: an in vitro approach using a continuous culture colonic model system

S Maccaferri, B Vitali, A Klinder, S Kolida…�- Journal of�…, 2010 - academic.oup.com
three-stage continuous culture system is a useful tool to monitor the ecology and metabolic
… (900 mL) and the retention time (36 h) of the colonic model system, 280 mg of rifaximin was …