The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set
In situ hybridization with rRNA-targeted oligonucleotide probes has become a widely
applied tool for direct analysis of microbial population structures of complex natural and
engineered systems. In such studies probe EUB338 (Amann et al., 1990) is routinely used to
quantify members of the domain Bacteria with a sufficiently high cellular ribosome content.
Recent reevaluations of probe EUB338 coverage based on all publicly available 16S rRNA
sequences, however, indicated that important bacterial phyla, most notably the�…
applied tool for direct analysis of microbial population structures of complex natural and
engineered systems. In such studies probe EUB338 (Amann et al., 1990) is routinely used to
quantify members of the domain Bacteria with a sufficiently high cellular ribosome content.
Recent reevaluations of probe EUB338 coverage based on all publicly available 16S rRNA
sequences, however, indicated that important bacterial phyla, most notably the�…
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