Insulin and contraction directly stimulate UCP2 and UCP3 mRNA expression in rat skeletal muscle in vitro
SB Pedersen, S Lund, ES Buhl, B Richelsen�- Biochemical and Biophysical�…, 2001 - Elsevier
To study the regulation of the mitochondrial uncoupling protein 2 and 3 (UCP2 and UCP3),
we studied the effect of insulin and muscle contraction on UCP mRNA expression in rat
skeletal muscle in vitro. Insulin dose-dependently increased skeletal muscle UCP2 and
UCP3 mRNA expression in m. extensor digitorum longus (EDL) with maximal stimulation
obtained at around 0.6–6 nM. The concentration of insulin giving half-maximal stimulation
was 60 pM for the UCP2 and 48 pM for the UCP3 mRNA expression. The effect of insulin�…
we studied the effect of insulin and muscle contraction on UCP mRNA expression in rat
skeletal muscle in vitro. Insulin dose-dependently increased skeletal muscle UCP2 and
UCP3 mRNA expression in m. extensor digitorum longus (EDL) with maximal stimulation
obtained at around 0.6–6 nM. The concentration of insulin giving half-maximal stimulation
was 60 pM for the UCP2 and 48 pM for the UCP3 mRNA expression. The effect of insulin�…
Regulation of UCP1, UCP2, and UCP3 mRNA expression in brown adipose tissue, white adipose tissue, and skeletal muscle in rats by estrogen
SB Pedersen, JM Bruun, K Kristensen…�- …�and biophysical research�…, 2001 - Elsevier
The effects of ovariectomy (OVX) and estrogen substitution on body weight, body
composition, food intake, weight gain, and expression of uncoupling proteins (UCPs) in
brown adipose tissue (BAT), white adipose tissue (WAT), and skeletal muscle were studied
in four groups of rats:(1) Sham-operated rats (N= 8),(2) ovariectomized rats (OVX− E)(N=
8),(3) estrogen-treated OVX rats (OVX+ E)(N= 8), and (4) OVX rats on energy restriction
(OVX− E+ D)(N= 8). OVX was associated with an increase in food intake and body weight�…
composition, food intake, weight gain, and expression of uncoupling proteins (UCPs) in
brown adipose tissue (BAT), white adipose tissue (WAT), and skeletal muscle were studied
in four groups of rats:(1) Sham-operated rats (N= 8),(2) ovariectomized rats (OVX− E)(N=
8),(3) estrogen-treated OVX rats (OVX+ E)(N= 8), and (4) OVX rats on energy restriction
(OVX− E+ D)(N= 8). OVX was associated with an increase in food intake and body weight�…
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