Sixteen oligonucleotide identification probes, designed in this study or adapted from
literature, were tested for a PCR-ELISA application to simultaneously detect under
standardised conditions selected intestinal bacteria, lactobacilli and bifidobacteria. The level
of specificity obtained with most of the probes fulfilled the set criteria. The lack of efficiency of
PCR performed with the primers, proposed to be specific for the entire eubacteria domain,
and compromises made in hybridisation conditions due to simultaneous usage of multiple�…