Optimisation of culture medium and conditions for α-smallcap˜L-arabinofuranosidase production by the extreme thermophilic eubacterium Rhodothermus marinus.
J Gomes, I Gomes, K Terler, N Gubala, G Ditzelm�ller… - 2000 - cabidigitallibrary.org
The culture medium for Rhodothermus marinus was optimised on a shake-flask scale by
using statistical factorial designs for enhanced production of a highly thermostable α-
smallcap˜ L-arabinofuranosidase (AFase). The medium containing 3.6 g/l birch wood xylan
and 8.2 g/l yeast extract yielded a maximum of 110 nkat/ml AFase activity together with 125
nkat/ml xylanase and 65 nkat/ml β-xylosidase activity. In addition, low levels of β-
mannanase (30 nkat/ml), α-galactosidase (0.2 nkat/ml), β-galactosidase (0.3 nkat/ml)�…
using statistical factorial designs for enhanced production of a highly thermostable α-
smallcap˜ L-arabinofuranosidase (AFase). The medium containing 3.6 g/l birch wood xylan
and 8.2 g/l yeast extract yielded a maximum of 110 nkat/ml AFase activity together with 125
nkat/ml xylanase and 65 nkat/ml β-xylosidase activity. In addition, low levels of β-
mannanase (30 nkat/ml), α-galactosidase (0.2 nkat/ml), β-galactosidase (0.3 nkat/ml)�…
Optimisation of culture medium and conditions for α-L-arabinofuranosidase production by the extreme thermophilic eubacterium Rhodothermus marinus
J Gomes, I Gomes, K Terler, N Gubala…�- Enzyme and microbial�…, 2000 - Elsevier
The culture medium for Rhodothermus marinus was optimised on a shake-flask scale by
using statistical factorial designs for enhanced production of a highly thermostable α-l-
arabinofuranosidase (AFase). The medium containing 3.6 g/l birch wood xylan and 8.2 g/l
yeast extract yielded a maximum of 110 nkat/ml AFase activity together with 125 nkat/ml
xylanase and 65 nkat/ml β-xylosidase activity. In addition, low levels of β-mannanase (30
nkat/ml), α-galactosidase (0.2 nkat/ml), β-galactosidase (0.3 nkat/ml), endoglucanase (5�…
using statistical factorial designs for enhanced production of a highly thermostable α-l-
arabinofuranosidase (AFase). The medium containing 3.6 g/l birch wood xylan and 8.2 g/l
yeast extract yielded a maximum of 110 nkat/ml AFase activity together with 125 nkat/ml
xylanase and 65 nkat/ml β-xylosidase activity. In addition, low levels of β-mannanase (30
nkat/ml), α-galactosidase (0.2 nkat/ml), β-galactosidase (0.3 nkat/ml), endoglucanase (5�…
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