Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
Y Sugiyama, I Kawabata, K Sobue, S Okabe�- Nature methods, 2005 - nature.com
To build a quantitative model of molecular organization of neurons, it is essential to have
information about the number of protein molecules at individual synapses. Here we
developed a method to estimate absolute numbers of individual proteins at actual excitatory
synapses by calibrating the fluorescence intensity of microspheres with single EGFP
molecules. In cultured hippocampal neurons, we observed a monotonous increase of
postsynaptic protein numbers per single synapse during neuronal differentiation and�…
information about the number of protein molecules at individual synapses. Here we
developed a method to estimate absolute numbers of individual proteins at actual excitatory
synapses by calibrating the fluorescence intensity of microspheres with single EGFP
molecules. In cultured hippocampal neurons, we observed a monotonous increase of
postsynaptic protein numbers per single synapse during neuronal differentiation and�…
[CITATION][C] Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
杉山佳子 - 2005 - ci.nii.ac.jp
CiNii 博士論文 - Determination of absolute protein numbers in single synapses by a GFP-based
calibration technique … Determination of absolute protein numbers in single synapses by a
GFP-based calibration technique 単一海馬興奮性シナプスにおけるシナプス後部分子絶対数の
測定 … Determination of absolute protein numbers in single synapses by a GFP-based
calibration technique …
calibration technique … Determination of absolute protein numbers in single synapses by a
GFP-based calibration technique 単一海馬興奮性シナプスにおけるシナプス後部分子絶対数の
測定 … Determination of absolute protein numbers in single synapses by a GFP-based
calibration technique …
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