[HTML][HTML] Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites
Skeletal muscle, 2013•Springer
Background Transcription factor overexpression is common in biological experiments and
transcription factor amplification is associated with many cancers, yet few studies have
directly compared the DNA-binding profiles of endogenous versus overexpressed
transcription factors. Methods We analyzed MyoD ChIP-seq data from C2C12 mouse
myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells
by overexpression of MyoD and compared the genome-wide binding profiles and binding�…
transcription factor amplification is associated with many cancers, yet few studies have
directly compared the DNA-binding profiles of endogenous versus overexpressed
transcription factors. Methods We analyzed MyoD ChIP-seq data from C2C12 mouse
myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells
by overexpression of MyoD and compared the genome-wide binding profiles and binding�…
Background
Transcription factor overexpression is common in biological experiments and transcription factor amplification is associated with many cancers, yet few studies have directly compared the DNA-binding profiles of endogenous versus overexpressed transcription factors.
Methods
We analyzed MyoD ChIP-seq data from C2C12 mouse myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells by overexpression of MyoD and compared the genome-wide binding profiles and binding site characteristics of endogenous and overexpressed MyoD.
Results
Overexpressed MyoD bound to the same sites occupied by endogenous MyoD and possessed the same E-box sequence preference and co-factor site enrichments, and did not bind to new sites with distinct characteristics.
Conclusions
Our data demonstrate a robust fidelity of transcription factor binding sites over a range of expression levels and that increased amounts of transcription factor increase the binding at physiologically bound sites.
Springer
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