[PDF][PDF] Identification and characterisation of transcription factors required for Dictyostelium prestalk differentiation
H SENOO - 2012 - iwate-u.repo.nii.ac.jp
Dictyosteliumdiscoideum, thesocialamoeba, isa widelyusedmodelsystem lorstudyinga
variety01processesindevelopment, includingcell-cellsignaling, signaltransduction,
patternlormationandthemovement01tissue-likeaggregates01 cells. Inmulticellularorganism,
organiserplaysanessentialroleindevelopment. As a delinition, ithasbeenproposedthatorganiserdetermine…
withothergermregion. AlthoughthesocialamoebaDictyosteliumdisc… beenknowntohavesimilarcharacteristicsinth…
, itsdevelopmentallunctionispoorlyunderstand. Duringmulticellulardevelopment01theD�…
variety01processesindevelopment, includingcell-cellsignaling, signaltransduction,
patternlormationandthemovement01tissue-likeaggregates01 cells. Inmulticellularorganism,
organiserplaysanessentialroleindevelopment. As a delinition, ithasbeenproposedthatorganiserdetermine…
withothergermregion. AlthoughthesocialamoebaDictyosteliumdisc… beenknowntohavesimilarcharacteristicsinth…
, itsdevelopmentallunctionispoorlyunderstand. Duringmulticellulardevelopment01theD�…
[HTML][HTML] An orthologue of the Myelin-gene Regulatory Transcription Factor regulates Dictyostelium prestalk differentiation
The prestalk region of the Dictyostelium slug is comprised of an anterior population of pstA
cells and a posterior population of pstO cells. They are distinguished by their ability to utilize
different parts of the promoter of the ecmA gene. We identify, by mutational analysis and
DNA transformation, CA-rich sequence elements within the ecmA promoter that are
essential for pstA-specific expression and sufficient to direct pstA-specific expression when
multimerised. The CA-rich region was used in affinity chromatography with nuclear extracts�…
cells and a posterior population of pstO cells. They are distinguished by their ability to utilize
different parts of the promoter of the ecmA gene. We identify, by mutational analysis and
DNA transformation, CA-rich sequence elements within the ecmA promoter that are
essential for pstA-specific expression and sufficient to direct pstA-specific expression when
multimerised. The CA-rich region was used in affinity chromatography with nuclear extracts�…
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