Abstract
Functional magnetic resonance imaging (fMRI) is a non-invasive technique that is widely available and can be used to determine the spatial relationships between tumor tissue and eloquent brain areas. Within certain limits, this functional information can be applied in the field of neurosurgery as a pre-operative mapping tool to minimize damage to eloquent brain areas. In this article, we review the literature on the use of fMRI for neurosurgical planning. The issues addressed are: (1) stimulation paradigms, (2) the influence of tumors on the blood oxygenation level-dependent (BOLD) signal, (3) post-processing the fMRI time course, (4) integration of fMRI results into neuronavigation systems, (5) the accuracy of fMRI and (6) fMRI compared to intra-operative mapping (IOM).
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Naidich TP, Grant JL, Altman N, Zimmerman RA, Birchansky SB, Braffman B, Daniel JL (1994) The developing cerebral surface. Preliminary report on the patterns of sulcal and gyral maturation-anatomy, ultrasound, and magnetic resonance imaging. Neuroimaging Clin N Am 4:201–240
Quinones-Hinojosa A, Ojemann SG, Sanai N, Dillon WP, Berger MS (2003) Preoperative correlation of intraoperative cortical mapping with magnetic resonance imaging landmarks to predict localization of the Broca area. J Neurosurg 99:311–318
Krings T, Reinges MH, Erberich S, Kemeny S, Rohde V, Spetzger U, Korinth M, Willmes K, Gilsbach JM, Thron A (2001) Functional MRI for presurgical planning: problems, artefacts, and solution strategies. J Neurol Neurosurg Psychiatry 70:749–760
Maldjian J, Atlas SW, Howard RS, Greenstein E, Alsop D, Detre JA, Listerud J, D’Esposito M, Flamm ES (1996) Functional magnetic resonance imaging of regional brain activity in patients with intracerebral arteriovenous malformations before surgical or endovascular therapy. J Neurosurg 84:477–483
Carpentier AC, Constable RT, Schlosser MJ, de Lotbiniere A, Piepmeier JM, Spencer DD, Awad IA (2001) Patterns of functional magnetic resonance imaging activation in association with structural lesions in the rolandic region: a classification system. J Neurosurg 94:946–954
Belliveau JW, Rosen BR, Kantor HL, Rzedzian RR, Kennedy DN, McKinstry RC, Vevea JM, Cohen MS, Pykett IL, Brady TJ (1990) Functional cerebral imaging by susceptibility-contrast NMR. Magn Reson Med 14:538–546
Ogawa S, Menon RS, Tank DW, Kim SG, Merkle H, Ellermann JM, Ugurbil K (1993) Functional brain mapping by blood oxygenation level-dependent contrast magnetic resonance imaging. A comparison of signal characteristics with a biophysical model. Biophys J 64:803–812
DeYoe EA, Bandettini P, Neitz J, Miller D, Winans P (1994) Functional magnetic resonance imaging (FMRI) of the human brain. J Neurosci Methods 54:171–187
Heeger DJ, Ress D (2002) What does fMRI tell us about neuronal activity? Nat Rev Neurosci 3:142–151
Toronov V, Walker S, Gupta R, Choi JH, Gratton E, Hueber D, Webb A (2003) The roles of changes in deoxyhemoglobin concentration and regional cerebral blood volume in the fMRI BOLD signal. Neuroimage 19:1521–1531
Latchaw RE, Hu X, Ugurbil K, Hall WA, Madison MT, Heros RC (1995) Functional magnetic resonance imaging as a management tool for cerebral arteriovenous malformations. Neurosurgery 37:619–625
Stapleton SR, Kiriakopoulos E, Mikulis D, Drake JM, Hoffman HJ, Humphreys R, Hwang P, Otsubo H, Holowka S, Logan W, Rutka JT (1997) Combined utility of functional MRI, cortical mapping, and frameless stereotaxy in the resection of lesions in eloquent areas of brain in children. Pediatr Neurosurg 26:68–82
Dymarkowski S, Sunaert S, Oostende SV, Hecke PV, Wilms G, De-maerel P, Nuttin B, Plets C, Marchal G (1998) Functional MRI of the brain: localisation of eloquent cortex in focal brain lesion therapy. Eur Radiol 8:1573–1580
Lee CC, Ward HA, Sharbrough FW, Meyer FB, Marsh WR, Raffel C, So EL, Cascino GD, Shin C, Xu Y, Riederer SJ, Jack CR (1999) Assessment of functional MR imaging in neurosurgical planning. Am J Neuroradiol 20:1511–1519
Righini A, de Divitiis O, Prinster A, Spagnoli D, Appollonio I, Bello L, Scifo P, Tomei G, Villani R, Fazio F, Leonardi M (1996) Functional MRI: primary motor cortex localization in patients with brain tumors. J Comput Assist Tomogr 20:702–708
Atlas SW, Howard RS, Maldjian J, Alsop D, Detre JA, Listerud J, D’Esposito M, Judy KD, Zager E, Stecker M (1996) Functional magnetic resonance imaging of regional brain activity in patients with intracerebral gliomas: findings and implications for clinical management. Neurosurgery 38:329–338
Debus J, Essig M, Schad LR, Wenz F, Baudendistel K, Knopp MV, Engenhart R, Lorenz WJ (1996) Functional magnetic resonance imaging in a stereotactic setup. Magn Reson Imaging 14:1007–1012
Mueller WM, Yetkin FZ, Hammeke TA, Morris GL, Swanson SJ, Reichert K, Cox R, Haughton VM (1996) Functional magnetic resonance imaging mapping of the motor cortex in patients with cerebral tumors. Neurosurgery 39:515–520
Pujol J, Conesa G, Deus J, Vendrell P, Isamat F, Zannoli G, Marti-Vilalta JL, Capdevila A (1996) Presurgical identification of the primary sensorimotor cortex by functional magnetic resonance imaging. J Neurosurg 84:7–13
Achten E, Jackson GD, Cameron JA, Abbott DF, Stella DL, Fabinyi GC (1999) Presurgical evaluation of the motor hand area with functional MR imaging in patients with tumors and dysplastic lesions. Radiology 210:529–538
Hirsch J, Ruge MI, Kim KH, Correa DD, Victor JD, Relkin NR, Labar DR, Krol G, Bilsky MH, Souweidane MM, DeAngelis LM, Gutin PH (2000) An integrated functional magnetic resonance imaging procedure for preoperative mapping of cortical areas associated with tactile, motor, language, and visual functions. Neurosurgery 47:711–721
Holodny AI, Schulder M, Liu WC, Wolko J, Maldjian JA, Kalnin AJ (2000) The effect of brain tumors on BOLD functional MR imaging activation in the adjacent motor cortex: implications for image-guided neurosurgery. Am J Neuroradiol 21:1415–1422
Hill DL, Smith AD, Simmons A, Maurer JR, Cox TC, Elwes R, Brammer M, Hawkes DJ, Polkey CE (2000) Sources of error in comparing functional magnetic resonance imaging and invasive electrophysiological recordings. J Neurosurg 93:214–223
Roberts TP, Disbrow EA, Roberts HC, Rowley HA (2000) Quantification and reproducibility of tracking cortical extent of activation by use of functional MR imaging and magnetoencephalography. Am J Neuroradiol 21:1377–1387
Hoeller M, Krings T, Reinges MH, Hans FJ, Gilsbach JM, Thron A (2002) Movement artefacts and MR BOLD signal increase during different paradigms for mapping the sensorimotor cortex. Acta Neurochir (Wien) 144:279–284
Nelson L, Lapsiwala S, Haughton VM, Noyes J, Sadrzadeh AH, Moritz CH, Meyerand ME, Badie B (2002) Preoperative mapping of the supplementary motor area in patients harboring tumors in the medial frontal lobe. J Neurosurg 97:1108–1114
Sabbah P, Foehrenbach H, Dutertre G, Nioche C, DeDreuille O, Bellegou N, Mangin JF, Leveque C, Faillot T, Gaillard JF, Desgeorges M, Cordoliani YS (2002) Multimodal anatomic, functional, and metabolic brain imaging for tumor resection. Clin Imaging 26:6–12
Wilkinson ID, Romanowski CA, Jellinek DA, Morris J, Griffiths PD (2003) Motor functional MRI for pre-operative and intraoperative neurosurgical guidance. Br J Radiol 76:98–103
Kamada K, Houkin K, Takeuchi F, Ishii N, Ikeda J, Sawamura Y, Kuriki S, Kawaguchi H, Iwasaki Y (2003) Visualization of the eloquent motor system by integration of MEG, functional, and anisotropic diffusion-weighted MRI in functional neuronavigation. Surg Neurol 59:352–361
Ulmer JL, Krouwer HG, Mueller WM, Ugurel MS, Kocak M, Mark LP (2003) Pseudo-reorganization of language cortical function at fMR imaging: a consequence of tumor-induced neurovascular uncoupling. Am J Neuroradiol 24:213–217
Yousry TA, Schmid UD, Jassoy AG, Schmidt D, Eisner WE, Reulen HJ, Reiser MF, Lissner J (1995) Topography of the cortical motor hand area: prospective study with functional MR imaging and direct motor mapping at surgery. Radiology 195:23–29
Pujol J, Conesa G, Deus J, Lopez-Obarrio L, Isamat F, Capdevila A (1998) Clinical application of functional magnetic resonance imaging in presurgical identification of the central sulcus. J Neurosurg 88:863–869
Nimsky C, Ganslandt O, Kober H, Moller M, Ulmer S, Tomandl B, Fahlbusch R (1999) Integration of functional magnetic resonance imaging supported by magnetoencephalography in functional neuronavigation. Neurosurgery 44:1249–1255
Roux FE, Boulanouar K, Ranjeva JP, Tremoulet M, Henry P, Manelfe C, Sabatier J, Berry I (1999) Usefulness of motor functional MRI correlated to cortical mapping in Rolandic low-grade astrocytomas. Acta Neurochir (Wien) 141:71–79
Roux FE, Boulanouar K, Ibarrola D, Tremoulet M, Chollet F, Berry I (2000) Functional MRI and intraoperative brain mapping to evaluate brain plasticity in patients with brain tumours and hemiparesis. J Neurol Neurosurg Psychiatry 69:453–463
Krainik A, Lehericy S, Duffau H, Vlaicu M, Poupon F, Capelle L, Cornu P, Clemenceau S, Sahel M, Valery CA, Boch AL, Mangin JF, Bihan DL, Marsault C (2001) Role of the supplementary motor area in motor deficit following medial frontal lobe surgery. Neurology 57:871–878
Kober H, Nimsky C, Moller M, Hastreiter P, Fahlbusch R, Ganslandt O (2001) Correlation of sensorimotor activation with functional magnetic resonance imaging and magnetoencephalography in presurgical functional imaging: a spatial analysis. Neuroimage 14:1214–1228
Yetkin FZ, Mueller WM, Morris GL, McAuliffe TL, Ulmer JL, Cox RW, Daniels DL, Haughton VM (1997) Functional MR activation correlated with intraoperative cortical mapping. Am J Neuroradiol 18:1311–1315
Preibisch C, Pilatus U, Bunke J, Hoogenraad F, Zanella F, Lanfermann H (2003) Functional MRI using sensitivity-encoded echo planar imaging (SENSE-EPI). Neuroimage 19:412–421
Lehericy S, Duffau H, Cornu P, Capelle L, Pidoux B, Carpentier A, Auliac S, Clemenceau S, Sichez JP, Bitar A, Valery CA, Effenterre RV, Faillot T, Srour A, Fohanno D, Philippon J, Bihan DL, Marsault C (2000) Correspondence between functional magnetic resonance imaging somatotopy and individual brain anatomy of the central region: comparison with intraoperative stimulation in patients with brain tumors. J Neurosurg 92:589–598
Roux FE, Ibarrola D, Tremoulet M, Lazorthes Y, Henry P, Sol JC, Berry I (2001) Methodological and technical issues for integrating functional magnetic resonance imaging data in a neuronavigational system. Neurosurgery 49:1145–1156
Fandino J, Kollias SS, Wieser HG, Valavanis A, Yonekawa Y (1999) Intraoperative validation of functional magnetic resonance imaging and cortical reorganization patterns in patients with brain tumors involving the primary motor cortex. J Neurosurg 91:238–250
Rutten GJ, van Rijen PC, van Veelen CW, Ramsey NF (1999) Language area localization with three-dimensional functional magnetic resonance imaging matches intrasulcal electrostimulation in Broca’s area. Ann Neurol 46:405–408
Roux FE, Boulanouar K, Lotterie JA, Mejdoubi M, LeSage JP, Berry I (2003) Language functional magnetic resonance imaging in preoperative assessment of language areas: correlation with direct cortical stimulation. Neurosurgery 52:1335–1345
Rutten GJ, Ramsey NF, van Rijen PC, Noordmans HJ, van Veelen CW (2002) Development of a functional magnetic resonance imaging protocol for intraoperative localization of critical temporoparietal language areas. Ann Neurol 51:350–360
Krainik A, Lehericy S, Duffau H, Capelle L, Chainay H, Cornu P, Cohen L, Boch AL, Mangin JF, Bihan DL, Marsault C (2003) Postoperative speech disorder after medial frontal surgery: role of the supplementary motor area. Neurology 60:587–594
Gumprecht H, Ebel GK, Auer DP, Lumenta CB (2002) Neuronavigation and functional MRI for surgery in patients with lesion in eloquent brain areas. Minim Invasive Neurosurg 45:151–153
Lurito JT, Lowe MJ, Sartorius C, Mathews VP (2000) Comparison of fMRI and intraoperative direct cortical stimulation in localization of receptive language areas. J Comput Assist Tomogr 24:99–105
Rombouts SA, Barkhof F, Hoogenraad FG, Sprenger M, Valk J, Scheltens P (1997) Test–retest analysis with functional MR of the activated area in the human visual cortex. Am J Neuroradiol 18:1317–1322
Lee YJ, Chung TS, Yoon YS, Lee MS, Han SH, Seong GJ, Ahn KJ (2001) The role of functional MR imaging in patients with ischemia in the visual cortex. Am J Neuroradiol 22:1043–1049
Vlieger EJ, Lavini C, Majoie CB, den Heeten GJ (2003) Reproducibility of functional MR imaging results using two different MR systems. Am J Neuroradiol 24:652–657
Sereno M, Dale A, Reppas J, Kwong K, Belliveau J, Brady T, Rosen B, Tootell R (1995) Borders of multiple visual areas in humans revealed by functional magnetic resonance imaging. Science 268:889–893
Tootell R, Mendola J, Hadjikhani N, Ledden P, Liu A, Reppas J, Sereno M, Dale A (1997) Functional analysis of V3A and related areas in human visual cortex. J Neurosci 17:7060–7078
Herholz K, Reulen HJ, von Stockhausen HM, Thiel A, Ilmberger J, Kessler J, Eisner W, Yousry TA, Heiss WD (1997) Preoperative activation and intraoperative stimulation of language-related areas in patients with glioma. Neurosurgery 41:1253–1260
Olivier A (1996) Surgical strategies for patients with supplementary sensorimotor area epilepsy. The Montreal experience. Adv Neurol 70:429–443
Zentner J, Hufnagel A, Pechstein U, Wolf HK, Schramm J (1996) Functional results after resective procedures involving the supplementary motor area. J Neurosurg 85:542–549
Skirboll SS, Ojemann GA, Berger MS, Lettich E, Winn HR (1996) Functional cortex and subcortical white matter located within gliomas. Neurosurgery 38:678–684
Schiffbauer H, Ferrari P, Rowley HA, Berger MS, Roberts TP (2001) Functional activity within brain tumors: a magnetic source imaging study. Neurosurgery 49:1313–1320
Gustard S, Williams EJ, Hall LD, Pickard JD, Carpenter TA (2003) Influence of baseline hematocrit on between-subject BOLD signal change using gradient echo and asymmetric spin echo EPI. Magn Reson Imaging 21:599–607
Cohen ER, Ugurbil K, Kim SG (2002) Effect of basal conditions on the magnitude and dynamics of the blood oxygenation level-dependent fMRI response. J Cereb Blood Flow Metab 22:1042–1053
Laurienti PJ, Field AS, Burdette JH, Maldjian JA, Yen YF, Moody DM (2002) Dietary caffeine consumption modulates fMRI measures. Neuroimage 17:751–757
Lee JH, Telang FW, Springer C, Volkow ND (2003) Abnormal brain activation to visual stimulation in cocaine abusers. Life Sci 73:1953–1961
Buhmann C, Glauche V, Sturenburg HJ, Oechsner M, Weiller C, Buchel C (2003) Pharmacologically modulated fMRI-cortical responsiveness to levodopa in drug-naive hemiparkinsonian patients. Brain 126:451–461
Schreiber A, Hubbe U, Ziyeh S, Hennig J (2000) The influence of gliomas and nonglial space-occupying lesions on blood-oxygen-level-dependent contrast enhancement. Am J Neuroradiol 21:1055–1063
Krings T, Topper R, Willmes K, Reinges MH, Gilsbach JM, Thron A (2002) Activation in primary and secondary motor areas in patients with CNS neoplasms and weakness. Neurology 58:381–390
Pouratian N, Sheth S, Bookheimer SY, Martin NA, Toga AW (2003) Applications and limitations of perfusion-dependent functional brain mapping for neurosurgical guidance. Neurosurg Focus 15:1–8
Ojemann JG, Miller JW, Silbergeld DL (1996) Preserved function in brain invaded by tumor. Neurosurgery 39:253–258
Jenkinson M, Bannister P, Brady M, Smith S (2002) Improved optimization for the robust and accurate linear registration and motion correction of brain images. Neuroimage 17:825–841
Liu H, Hall WA, Truwit CL (2003) The roles of functional MRI in MR-guided neurosurgery in a combined 1.5 Tesla MR-operating room. Acta Neurochir [Suppl] 85:127–135
Friston KJ, Holmes AP, Worsley KJ, Poline JP, Frith CD, Frackowiak RS (1995) Statistical parametric maps in functional imaging: a general linear approach. Hum Brain Mapp 2:189–210
Friston KJ, Worsley KJ, Frackowiak RS, Mazziotta JC, Evans AC (1994) Assessing the significance of focal activations using their spatial extent. Hum Brain Mapp 1:214–220
Maldjian JA, Schulder M, Liu WC, Mun IK, Hirschorn D, Murthy R, Carmel P, Kalnin A (1997) Intraoperative functional MRI using a real-time neurosurgical navigation system. J Comput Assist Tomogr 21:910–912
McDonald JD, Chong BW, Lewine JD, Jones G, Burr RB, McDonald PR, Koehler SB, Tsuruda J, Orrison WW, Heilbrun MP (1999) Integration of preoperative and intraoperative functional brain mapping in a frameless stereotactic environment for lesions near eloquent cortex. Technical note. J Neurosurg 90:591–598
Krings T, Schreckenberger M, Rohde V, Foltys H, Spetzger U, Sabri O, Reinges MH, Kemeny S, Meyer PT, Moller-Hartmann W, Korinth M, Gilsbach JM, Buell U, Thron A (2001) Metabolic and electrophysiological validation of functional MRI. J Neurol Neurosurg Psychiatry 71:762–771
Viergever MA, Maintz JB, Niessen WJ, Noordmans HJ, Pluim JP, Stokking R, Vincken KL (2001) Registration, segmentation, and visualization of multimodal brain images. Comput Med Imaging Graph 25:147–151
Maldjian JA, Listerud J, Khalsa S (2002) Integrating postprocessed functional MR images with picture archiving and communication systems. Am J Neuroradiol 23:1393–1397
Yetkin FZ, McAuliffe TL, Cox R, Haughton VM (1996) Test–retest precision of functional MR in sensory and motor task activation. Am J Neuroradiol 17:95–98
Cohen MS, DuBois RM (1999) Stability, repeatability, and the expression of signal magnitude in functional magnetic resonance imaging. J Magn Reson Imaging 10:33–40
McGonigle DJ, Howseman AM, Athwal BS, Friston KJ, Frackowiak RS, Holmes AP (2000) Variability in fMRI: an examination of intersession differences. Neuroimage 11:708–734
Miki A, Raz J, van Erp TG, Liu CS, Haselgrove JC, Liu GT (2000) Reproducibility of visual activation in functional MR imaging and effects of postprocessing. Am J Neuroradiol 21:910–915
Li T, Mirowitz S (2003) Fast T2-weighted MR imaging: impact of variation in pulse sequence parameters on image quality and artifacts. Magn Reson Imaging 21:745–753
Schmithorst VJ, Dardzinski BJ, Holland SK (2001) Simultaneous correction of ghost and geometric distortion artifacts in EPI using a multiecho reference scan. IEEE Trans Med Imaging 20:535–539
Jenkinson M (2001) Improved unwarping of EPI volumes using regularised B 0 maps. Human brain mapping, Brighton, UK; book of abstracts, abstract 11324
Hutton C, Bork A, Josephs O, Deichmann R, Ashburner J, Turner R (2002) Image distortion correction in fMRI: a quantitative evaluation. Neuroimage 16:217–240
Delon-Martin C, Roth M, Morand S, Massarelli R, Belle V, Decety J, Felblinger J, Decorps M, Segebarth C (1999) MRI measurement of the functional blood flow changes in a large superficial vein draining the motor cortex. NMR Biomed 12:107–114
Turner R (2002) How much cortex can a vein drain? Downstream dilution of activation-related cerebral blood oxygenation changes. Neuroimage 16:1062–1067
Hartkens T, Hill DL, Castellano-Smith AD, Hawkes DJ, Maurer CR, Martin AJ, Hall WA, Liu H, Truwit CL (2003) Measurement and analysis of brain deformation during neurosurgery. IEEE Trans Med Imaging 22:82–92
Nimsky C, Ganslandt O, Hastreiter P, Fahlbusch R (2001) Intraoperative compensation for brain shift. Surg Neurol 56:357–364
Jack JR, Thompson RM, Butts RK, Sharbrough FW, Kelly PJ, Hanson DP, Riederer SJ, Ehman RL, Hangiandreou NJ, Cascino GD (1994) Sensory motor cortex: correlation of presurgical mapping with functional MR imaging and invasive cortical mapping. Radiology 190:85–92
Haglund MM, Ojenmann GA, Hochaman DW (1992) Optical imaging of epileptiform and functional activity in human cerebral cortex. Nature 358:668–671
Smith SM (2002) Fast robust automated brain extraction. Hum Brain Mapp 17:143–155
Weiger M, Pruessmann KP, Osterbauer R, Bornert P, Boesiger P, Jezzard P (2002) Sensitivity-encoded single-shot spiral imaging for reduced susceptibility artifacts in BOLD fMRI. Magn Reson Med 48:860–866
Nimsky C, Ganslandt O, Kober H, Buchfelder M, Fahlbusch R (2001) Intraoperative magnetic resonance imaging combined with neuronavigation: a new concept. Neurosurgery 48:1082–1089
Ganslandt O, Behari S, Gralla J, Fahlbusch R, Nilsky C (2002) Neuronavigation: concept, techniques and applications. Neurol India 50:244–255
Clark C, Barrick T, Murphy M, Bell B (2003) White matter fiber tracking in patients with space-occupying lesions of the brain: a new technique for neurosurgical planning? Neuroimage 20:1601–1608
Mori S, Frederiksen K, van Zijl PC, Stieltjes B, Kraut MA, Solaiyappan M, Pomper MG (2002) Brain white matter anatomy of tumor patients evaluated with diffusion tensor imaging. Ann Neurol 51:377–380
Hendler T, Pianka P, Sigal M, Kafri M, Ben-Bashat D, Constantini S, Graif M, Fried I, Assaf Y (2003) Delineating gray and white matter involvement in brain lesions: three-dimensional alignment of functional magnetic resonance and diffusion-tensor imaging. J Neurosurg 99:1018–1027
Witwer B, Moftakhar R, Hasan K, Deshmukh P, Haugton V, Field A, Arfanakis K, Noyes J, Moritz C, Meyerand M, Rowley H, Alexander A, Badie B (2002) Diffusion-tensor imaging of white matter tracts in patients with cerebral neoplasm. J Neurosurg 97:568–575
Wang J, Aguirre GK, Kimberg DY, Detre JA (2003) Empirical analyses of null-hypothesis perfusion FMRI data at 1.5 and 4 T. Neuroimage 19:1449–1462
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The authors thank Cristina Lavini for her help with the implementation fMRI in our hospital, and Wendy van Noppen for her help with the preparation of this manuscript.
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Vlieger, EJ., Majoie, C.B., Leenstra, S. et al. Functional magnetic resonance imaging for neurosurgical planning in neurooncology. Eur Radiol 14, 1143–1153 (2004). https://doi.org/10.1007/s00330-004-2328-y
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DOI: https://doi.org/10.1007/s00330-004-2328-y